Heterotopic Transplantation of Posterior Pole Plasm in Drosophila Eggs (pp. 176-178)
Christian Sardet's web site contains contains all on localized cytoplasmic determinants in ascidians and other marine organisms. Many links, great images and movies!
p.173, left column, 3rd line from bottom, should read: "... the cyclic appearance, at the vegetal pole, ..."
Kloc M., Zearfoss N.R., and Etkin L.A. (2002) Mechanisms of subcellular mRNA localization. Cell 108: 533-544
Nishida H. (2002) Patterning the marginal zone of early ascidian embryos: localized maternal mRNAs and inductive interactions. BioEssays 24: 613-624
Nishida H. and Sawada K. (2001) macho-1 encodes a localized mRNA in ascidian eggs that specifies muscle fate during development. Nature 409: 724-729
The authors identified a localized mRNA, encoded maternally by the macho-1 gene, that acts as a localized cytoplasmic determinant for primary tail muscle in the egg of the ascidian Halocynthia roretzi. The distribution of macho-1 mRNA corresponds closely to that of yellow cytoplasm, which was shown earlier to act as a localized cytoplasmic determinant for the primary muscle cell lineage (see Fig. 8.15 on p.184 of text). Depletion of macho mRNA by injection of antisense deoxyoligonucleotides (see p. 69 and p. 218) interferes specifically with tail muscle formation, as indicated by the lack of muscle-specific marker proteins such as myosin and acetylcholinesterase. Such depleted embryos can be rescued by injecting normal macho mRNA but not by mRNA lacking a region that encodes a functional domain of the macho protein, which is a transcription factor of the zinc-finger type. Oversupply of macho mRNA leads to muscle formation in cell lineages that normally form non-muscle tissues. Thus, macho mRNA is a localized maternal mRNA that is necessary and sufficient for primary tail muscle formation.
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