Dr. Reichlerıs Bio 212 MWF 2-3pm Print Name:____________KEY____________
Exam #3 Nov. 11, 2003
Answer each question as succinctly as possible in the space provided. If needed, continue on the back. If you use a drawing as part of your answer, be sure to also include a written explanation. Read each question carefully and donıt hesitate to ask if a question seems unclear. These questions have specific answers, although for some, more than one answer is possible. To receive full credit you must clearly and fully answer the question being asked. Each question is worth 6 pts, unless otherwise noted, for a total of 103 points possible for this exam.
1. What is one piece of information that you would need to know about a gene to insert and/or express it in bacteria? How would you use this information to ensure that this gene would be inserted and/or expressed in the bacteria? (8 pts)
Any of: need to know the sequence so that you can choose a restriction enzyme or so PCR primers can be made. Need to know if there are introns so reverse transcription can be done if needed. Need to know about the promoter so that it can be matched with a bacterial promoter for expression.
Restriction enzymes are used to cut the plasmid and the gene of interest. Any one of: needs to not cut inside the gene, should cut in the lacZ gene for blue/white screening, must cut on both sides of the gene, should leave sticky ends for ligation.