FIGURE 1. Dictyostelium wild-type and racE null cells complete cytokinesis normally when attached to a substrate. Dictyostelium wild-type (strain DH1) and racE null (strain 24EH6) cells were allowed to attach to coverslips and were visualized by video-light microscopy. (A) Wild-type cells form a cleavage furrow that divides the cells 3-5 minutes after furrow formation. As the daughter cells separate, they form large pseudopods (white arrowheads) to migrate away from each other. (B) RacE null cells also form a cleavage furrow that successfully divides the cell. However, the daughter cells do not form large pseudopods. Instead, they have multiple filopodia (white arrowheads) and a broad leading edge (bracket). The time between each frame is 10 sec.