FIGURE 3. RacE null cells form blebs and fail to complete cytokinesis when grown in suspension culture. Wild-type and racE null cells were placed in a 0.03% agarose solution to maintain them in suspension conditions and were observed by video microscopy.
(A) Wild-type cells form a cleavage furrow that rapidly divides the cells. After cleavage is complete, the daughter cells attempt to migrate away from each other by the extension of pseudopods.
(B) These racE null cells also form a cleavage furrow but do not divide successfully. As the cells attempt to divide, they form blebs and their furrows regress.